Sizeable polymer plants “Orion Global PET” and Joint Stock Company “Neo Group” are operating in Lithuania along with the lubricant manufacturer “SCT Lubricants”, all being interested in local suppliers. As raw materials in plastics and lubricants, various esters, manufactured by traditional chemical methods, are often used. Manufacture of biodiesel, which is also an ester, is growing in Lithuania. Since it is subsidized by government, it is important to diversify this production and offer the technology for the products of higher value, because the biodiesel subsidies will be reduced and eliminated eventually. This project is devoted to development of new technologies for producing higher value added products utilizing biotechnological synthesis from oils, fats and other renewable resources. Most promising samples will be selected and new manufacturing technologies will be introduced for production and evaluation of pilot scale experimental products: low flammability ageing-resistant biodegradable esters. Esters’ suitability for two applications will be investigated: 1) as plasticizers, used in manufacture of plastics, and 2) as base oils, used in manufacture of biolubricants. Plasticizers influence not only mechanical elasticity and durability of plastics, but also their odor, toxicity in contact with food, flammability due to exposure to fire, and consumer safety. Base oils are used for manufacturing of lubricants by dissolving functional additives in them. They dictate most lubricant properties, among those flammability and resistance to ageing. Both plasticizers and biolubricant base oils are made from organic esters, manufactured using chemical methods. Broad experience of lipase-based synthesis is accumulated in Lithuania and can be used in biotechnological synthesis of esters. The process conditions will be selected in such manner that esters, produced using glycols, would target the most important technical requirements, specified for polymer plasticizers and biolubricant base oils. Transesterification reactions using glycols will be carried out, along with lipase-based modification of alkyl chains. Acyl chain structure will be controlled by choosing selective lipases, interacting differently with specific fatty acids. Also the investigations will involve screening for selectivity towards fatty acids by carrying out the biotechnological synthesis in emulsions, replacing lipases with their producers and concurrently evaluating the influence of emulsifiers on lipase inactivation. Such selectivity would give an opportunity to control the flammability of obtained biodegradable esters. In addition to the studies of synthesis directions, experimental methodologies will be developed with the purpose of rapid testing of material’s suitability for plasticizers and biolubricant base oils. Not only their flammability and ageing will be evaluated, but also the volatility, hydrolysis and interaction between comparison samples with functional additives and system components. Most promising directions of biotechnological synthesis will be supplemented with appropriate processing stages, which would improve technical parameters of biodegradable esters. It will be attempted to evaluate the quality of experimental production from the pilot scale manufacture by providing its samples to potential users. Developed technology of biodegradable ester production will be optimized by reducing the manufacturing costs. Research conclusions will be made available to interested organizations, including polymer, biodiesel and lubricant manufacturers in Lithuania.